AquaTech4FeedPhoto: Mark Lietze
[XX International symposium on fish nutrition and feeding towards precision fish nutrition and feeding - Sorrento, Italy - 5/9 June 2022]
The bioavailability of nutrients and efficiency of fish enzymes to hydrolyse dietary ingredients determines their nutritional quality. In this sense, species-specific in vitro digestibility assays represent very useful tools when evaluating the potential of specific feedstuffs as novel ingredients for using in aquafeeds. These assays would greatly contribute to the three Rs principle by refining the in vivo experimentation requested with animals during the preliminary evaluation of novel ingredients. Taking these considerations into account, the main objective of this work was the in vitro evaluation of duckweed (Lemna sp.), microalgae (Nannochloropsis sp.), dabberlocks (Alaria esculenta) and deffated insect meal (Tenebrio molitor), using as reference ingredient fishmeal. in vitro protein hydrolysis of the novel ingredients was carried out by means of an in vitro digestibility assay using rainbow trout (Oncorhynchus mykiss) digestive enzymes. During the course of the in vitro assay, samples
were withdrawn at different times to evaluate the degree of hydrolysis expressed as Coefficient of Protein Degradation (CPD), and to quantify amino acids released. Additionally, a quantification of the reducing sugars released by rainbow trout enzymes was performed. Results obtained showed that protein content ranged from 11% in A. esculenta to 55% in T. molitor. On the other hand, visualization of electrophoresis gels revealed a progressive hydrolysis of the different protein fractions until the end of the assay, represented by a decrease in the optical density of bands. In addition, total free amino acids released at the end of the in vitro assay ranged from 2g 100 g protein-1 in the case of A. esculenta to 6 g 100 g protein-1 in Nannochloropsis sp., being those values lower than those obtained for fishmeal (9 g 100 g protein-1). Finally, data obtained from the quantification of reducing sugars revealed a negligible capability of trout enzymes for hydrolysing the polysaccharides, particularly those contained in the cell wall.
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